Barkha Singh

About

I am currently a Post-Doctoral Fellow at the University of Newcastle, Australia, where my research focuses on defining the matrisome of human endometrial cancer and developing organoid models using endometrial cancer ECM-based hydrogels. My work involves detailed characterization, validation, imaging, and functional testing of these models. Additionally, I am developing cell-derived extracellular matrices and designing scaffolds aimed at vaginal regeneration and wound healing. My techniques include decellularizing human and bovine endometrial tissues, validating the decellularization process, forming hydrogels, growing 3D organoids, performing cell viability assays, and conducting mechanical characterization of hydrogels through rheology and viscometry, along with proteomic analyses.

I completed my Ph.D. at the Indian Institute of Technology Bombay from 2017 to 2022 under the guidance of Prof. Rohit Srivastava. My thesis, titled "Green and Scalable Synthesis of Plasmonic Inorganic Nanoconstructs for Cancer Theranostics and their Toxicity Assessment," involved synthesizing various inorganic nanomaterials and hybrids, including MXene, gold nanoshells, tungsten disulfide nanosheets, tungsten disulfide quantum dots, silica nanoparticles, polymeric nanoparticles, carbon/graphene sheets, and carbon/quantum dots. I characterized these nanoparticles with multiple techniques and applied them as cancer theranostic models, particularly for photothermal therapy, while also conducting comprehensive toxicity assessments in vitro and in vivo. I was honored to receive the “Naik and Rastogi Award for Excellence in Ph.D. Research" for 2021-23 for my work.

I earned my Bachelor’s degree in Biotechnology in 2015, working on the project "Expression, Purification, and Immunolocalization of Leishmania Proteins" under the supervision of Dr. Amogh Shastrabuddhe at the MSB Division, CSIR-CDRI, Lucknow. I then pursued an M.Tech in Nanotechnology at the Indian Institute of Technology Roorkee in 2017, where I worked on an Indo-UK project titled "Quantum Dots Conjugated Drug Delivery System Encapsulated in Biodegradable Polymer Microspheres," guided by Prof. Kaushik Pal. 

Publication

Hybrid nanoarchitectonics of ordered mesoporous C60–BCN with high surface area for supercapacitors and lithium-ion batteries

Rohan Bahadur, Gurwinder Singh, Zhixuan Li, Barkha Singh, Rohit Srivastava, Yasuhiro Sakamoto, Shery Chang, Ramaswamy Murugavel, Ajayan Vinu

Carbon, vol. 216, 2024, p. 118568

Preclinical Toxicity Assessment of Ti-Based MXene Nanomaterials for Advanced Theranostic Applications

Barkha Singh, Rohan Bahadur, Deepika Rai, Rohit Srivastava

Advanced Therapeutics, vol. 7, 2024, p. 2300268

Influence of PEGylation on WS2 Nanosheets and Its Application in Photothermal Therapy

Rohan Bahadur, Barkha Singh, Deepika Rai, Rohit Srivastava

ACS Applied Bio Materials, vol. 6, 2023, pp. 4740-4748

Fabrication and cytotoxicity evaluation of polyethyleneimine conjugated fluorescent MXene nanosheets as cancer theranostics agent

Barkha Singh, Rohan Bahadur, Mayuri Gandhi, Rohit Srivastava

Polymer Bulletin, vol. 80, Springer, 2023, pp. 11329--11342

Preclinical safety assessment of red emissive gold nanocluster conjugated crumpled MXene nanosheets: a dynamic duo for image-guided photothermal therapy

Barkha Singh, Rohan Bahadur, Priyanka Maske, Mayuri Gandhi, Dipty Singh, Rohit Srivastava

Nanoscale, vol. 15(6), The Royal Society of Chemistry, 2023, pp. 2932-2947

Synthesis and degradation mechanism of renally excretable gold core–shell nanoparticles for combined photothermal and photodynamic therapy

Barkha Singh, Chetna Patnaik, Rohan Bahadur, Mayuri Gandhi, Abhijit De, Rohit Srivastava

Nanoscale, vol. 15(3), The Royal Society of Chemistry, 2023, pp. 1273-1288

Preparation and characterization of PLA microspheres as drug delivery system for controlled release of Cetirizine with carbon dots as drug carrier

Barkha Singh, Swati Singh, Abhishek Gautam, Andrew Sutherland, Kaushik Pal

Polymer Bulletin, vol. 80, Springer, 2023, pp. 5741--5757

Designing and Immunomodulating Multiresponsive Nanomaterial for Cancer Theranostics

Amreen Khan, Faith Dias, Suditi Neekhra, Barkha Singh, Rohit Srivastava

Frontiers in Chemistry, vol. 8, 2021

Hydrothermal-Assisted Synthesis and Stability of Multifunctional MXene Nanobipyramids: Structural, Chemical, and Optical Evolution

Barkha Singh, Rohan Bahadur, Suditi Neekhra, Mayuri Gandhi, Rohit Srivastava

ACS Applied Materials \& Interfaces, vol. 13, 2021, pp. 3011-3023

Influence of Surface States on the Optical and Cellular Property of Thermally Stable Red Emissive Graphitic Carbon Dots

Barkha Singh, Rohan Bahadur, Misah Rangara, Mayuri N. Gandhi, Rohit Srivastava

ACS Applied Bio Materials, vol. 4, 2021, pp. 4641-4651

A biodegradable fluorescent nanohybrid for photo-driven tumor diagnosis and tumor growth inhibition

Rajendra Prasad, Deepak S. Chauhan, Amit S. Yadav, Janhavi Devrukhkar, Barkha Singh, Mahadeo Gorain, Mayur Temgire, Jayesh Bellare, Gopal C. Kundu, Rohit Srivastava

Nanoscale, vol. 10(40), The Royal Society of Chemistry, 2018, pp. 19082-19091

Control of shape and size of poly (lactic acid) microspheres based on surfactant and polymer concentration

Barkha Singh, Pushpendra Singh, Andrew J. Sutherland, Kaushik Pal

Materials Letters, vol. 195, 2017, pp. 48-51

Book Chapters

Application of Two-Dimensional Materials for Cancer Theranostic

Barkha Singh, Ritika Uday Gaitonde

Nanomaterials in Healthcare, CRC Press, 2024, pp. 259--279

EPR-Selective Biodegradable Polymer-Based Nanoparticles for Modulating ROS in the Management of Cervical Cancer

Sujit Kumar Debnath, Barkha Singh, Nidhi Agrawal, Rohit Srivastava

Sajal Chakraborti, Handbook of Oxidative Stress in Cancer: Therapeutic Aspects, Springer Nature Singapore, Singapore, 2022, pp. 2863--2889

Evolution Towards Theranostics: Basic Principles

Pallavi Kiran, Amreen Khan, Suditi Neekhra, Pankaj Kumar, Barkha Singh, Shubham Pallod, Faith Dias, Rohit Srivastava

Vivek Borse, Pranjal Chandra, Rohit Srivastava, BioSensing, Theranostics, and Medical Devices: From Laboratory to Point-of-Care Testing, Springer Singapore, Singapore, 2022, pp. 59--82

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